FIGURE 8.
Association of KREPB6-8 and KREN1-3 proteins with deletion and insertion subcomplexes, respectively. Expression of KREPA3 was repressed by RNAi (+) in PF cells in which either KREPB6, 7, or 8 or KREN1, 2, or 3 were TAP-tagged. Western analyses of (A) complexes after purification by IgG sepharose chromatography and TEV protease cleavage and (B–D) subsequent glycerol gradient fractionation. The blots were probed with α-His MAb against tagged proteins (A, top panel) or a mixture of MAbs against KREPA1, KREPA2, KREL1, and KREPA3 as indicated.
