Activities of KREN1 tagged editosomes from cells in which KREPB8 expression has been knocked down. (A) Western analyses of IgG purified editosomes from KREPB8 RNAi-N1TAP cells with KREPB8 expressed or repressed. The blots were probed with the editosome MAb mixture (bottom panel) or a MAb against calmodulin binding peptide (CBP) on the tagged KREN1 protein (top panel). In vitro pre-cleaved insertion (B), deletion (C) assays and full-round deletion editing assays (D). The input RNAs (Inp) are edited by addition of two U's (+2U) in the insertion assay, removal of four U's (−4U) in the deletion assay, and result in ligated products of unprocessed 5′ and 3′ input RNAs (Lig) and edited (Ed) products. Full round assays assess deletion cleavage activity (denoted by arrow). Other designations are as in Figure 4.
