FIGURE 5.
Effects of KREPB8 repression on editosome sedimentation and in vitro endoribonucleolytic cleavage activity of editosomes. (A) Western analysis of glycerol gradient fractions from crude mitochondrial lysates from KREPB8 RNAi cells comparing KREPB8 RNAi cells repressed for 6 d with nonrepressed cells as a control. The MAb mixture was used as described for Figure 4. Gradient fractions 11–19 were assayed for endonuclease activities that are specific for an insertion site cleaved by KREN2 (B) or deletion site cleaved by KREN1 (C). See legend for Figure 4 or Materials and Methods for details.
