Two examples of putative asRNAs in V. splendidus. (A) Visualization of the genomic context of Vsr285 on the Artemis viewer. Bam reads are visible on the top window and are highlighted in salmon. (Orange box) The Vsr285 encoding gene. (Yellow boxes) Putative transcription terminators, predicted by ARNold. (Bright pink) The r5′UTR of VS_II1110 encoding GuaC. (B) Analysis of expression of Vsr285 during growth of V. splendidus. Northern blotting was carried out as in Figure 4. (C) Vsr65 could be part of a type I toxin–antitoxin system. (Left) Genomic context of Vsr65 (orange box), transcribed antisense of VS_0930 (blue box). (Right) TMHMM profile of VS_0930 (see Materials and Methods) and amino acid sequence of the peptide. (Red) The two predicted transmembrane domains.
