Expression analysis of sRNAs by Northern blots. Total RNA samples were prepared from different time points during growth of V. splendidus in marine salt medium and submitted to electrophoresis on an 8% acrylamide gel before transfer to a Hybond N+ membrane. The culture OD600 for each sample is indicated above each lane, and the growth curve is presented underneath. Membranes were probed with [γ-32P]-labeled specific oligonucleotides for each candidate sRNA (Supplemental Table S2). The name of the sRNA is indicated on the right as well as the size of the transcript visualized in the RNA-seq. The right-most column indicates the presence of a transcription terminator predicted by ARNold (see Materials and Methods). (On the left) The approximate size determined by comparison with the migration of RNAs of known sizes. 4.5S RNA and tmRNA were used as loading controls.
