NMR structure of a 4 × 4 nucleotide RNA internal loop from an R2 retrotransposon: Identification of a three purine–purine sheared pair motif and comparison to MC-SYM predictions

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 2.
FIGURE 2.

NMR spectra of the internal loop from B. mori R2 retrotransposon 5′RNA in 90:10 (v/v) H2O:D2O at 0°C and pH 6.5. The imino proton region is shown. At top is the 1D spectrum; the middle presents two regions of a 2D SNOESY spectrum (mixing time = 100 msec); at bottom is a proton-nitrogen HSQC spectrum. The 22-mer model duplex is shown in the inset.

This Article

  1. Published in Advance July 21, 2011, doi: 10.1261/rna.2641911 RNA 17: 1664-1677

Current Issue

  1. August 2026, 32 (8)
  1. Current Issue
  1. Alert me to new issues of RNA