Pseudouridine formation in archaeal RNAs: The case of Haloferax volcanii

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 5.
FIGURE 5.

H. volcanii tRNA Ψ13, Ψ22, and Ψ39 are not Cbf5-mediated and Ψ39 is TruA/Pus3-mediated. (A) U-specific analyses to determine the modification status of U13 and U22 of elongator tRNAMet were done using primer HVMETCR2 and total tRNAs of WT and Δcbf5 strains. (Lanes 1,2) Primer extensions without and with U-specific reactions, respectively. Positions of certain Us and Ψ13 and Ψ22 in tRNA are indicated on the side. (B) CMCT-primer extension analyses to determine the modification status of U13 and U22 of tRNAMet were done using primer HVMETCR2 and total tRNAs of WT and Δcbf5 strains. Total tRNAs were treated with (+) or without (−) CMCT for the indicated time (in minutes), followed by alkali (OH−) treatment (+) or no treatment (−). Positions of Ψ13 and Ψ22 are indicated on the side. (C,D) Analysis similar to those in A and B, respectively, using primer VM5/6R, to determine the modification status of U39 of tRNAMet in WT (H26), Δpus3 (VDC2465), and Δcbf5 (VDC2364) strains.

This Article

  1. Published in Advance May 31, 2011, doi: 10.1261/rna.2712811 RNA 17: 1367-1380

Current Issue

  1. July 2026, 32 (7)
  1. Current Issue
  1. Alert me to new issues of RNA