Pseudouridine formation in archaeal RNAs: The case of Haloferax volcanii

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FIGURE 4.
FIGURE 4.

pus10 is essential in H. volcanii. pus10 (HVO_1979) could only be deleted from the chromosome when expressed in trans. (A) H26 (WT) and two independent isolates VDC2606 and VDC2607 (ΔHVO_1979 with plasmid-borne copy of HVO_1979 under Ptna control) were serially diluted from 100 to 10−4 and spotted onto Hv-Min media plates, plus and minus 500 μM tryptophan. Significant growth of the mutant is only observed in the presence of tryptophan. (B) Deletion of the chromosomal copy of HVO_1979 was further verified by PCR using primers Pus10_Ext_Fwd and Pus10_Ext_Rev. Predicted sizes of the amplicon are indicated above each band.

This Article

  1. Published in Advance May 31, 2011, doi: 10.1261/rna.2712811 RNA 17: 1367-1380

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