Pseudouridine formation in archaeal RNAs: The case of Haloferax volcanii

cbf5 deletion does not significantly reduce the amount of Ψ in H. volcanii total tRNAs and at tRNA position 55. Nuclease P1 digests of uniformly labeled tRNAs were resolved by thin layer chromatography. pA, pC, pG, pU, pΨ, and pm¹Ψ indicate 5′-phosphorylated A, C, G, U, Ψ, and m¹Ψ, respectively. (A,B) Digests of total tRNA and tRNA^Trp, respectively, from WT (upper panels) and Δcbf5 strains (lower panels). Ratio of Ψ and m¹Ψ is indicated in each panel.