The C-terminal end of the Trypanosoma brucei editing deaminase plays a critical role in tRNA binding

TbADAT2 C-terminal mutant still heterodimerizes with ADAT3. (A) Wild-type TbADAT2/3 and the two C-terminal mutants were fractionated by size-exclusion chromatography as indicated in the Materials and Methods section. Both mutants eluted with a size consistent with that calculated for the wild type (∼68 kDa), again suggesting that these mutations cause no major effects on the multimeric state of these mutants. (B) K_av vs. molecular weight standard curve. The standards and their respective molecular weights are as marked (gray diamonds) and labeled in gray. The wild-type and mutant ADAT2/3 enzymes are marked (black circles) and labeled in black.