Sharing and archiving nucleic acid structure mapping data

Example meta-analysis of DMS (OBI:0001015) chemical mapping data from two separate studies on RNA. For example, to visualize the effects of Mg²⁺ on the DMS reactivity of nucleic acids, we searched for OBI:0001015 (DMS) and CHEBI:6636 (MgCl₂) in ISA-Tab (Rocca-Serra et al. 2010) files and identified two studies where RNA was probed with DMS in the absence and presence of Mg²⁺. We then downloaded the two ISA-Tab files (https://spreadsheets.google.com/ccc?key=0As58Pw6ZT3UtdGFveExsek9tdUJNS0xXbUFmRE1ZR0E&hl=en#gid=1 and https://spreadsheets.google.com/ccc?key=0AvCayBYdTclldEJoQ3otbWE5RGx0VzdobmVjX2Q5b3c&hl=en#gid=0) and used a tool included in the SAFA software (Simmons et al. 2009) to visualize the data on the RNA. (A) Secondary structure diagram of the L-21 T. thermophila group I intron with DMS data mapped to its secondary structure with (right) and without (left) Mg²⁺ present. (B) Secondary structure diagram of domain IV of SRP with and without Mg²⁺ present.