FIGURE 2.
Sample preparation for SAXS experiments. (A) Chromatographic profiles of RNA samples prepared by different techniques. RNA from A. tumefaciens (“Atu”), B. stearothermophilus (“Bst”), or E. coli (“Eco”) was prepared by refolding from denatured state (“annealed”), nondenaturing method according to Batey and Kieft (2007) (“native”) or by transcription at elevated ionic strength and nondenaturing purification (“HS native”), and analyzed by high-resolution gel-filtration chromatography on Shodex KW 803. Absorbance at 254 nm is plotted versus retention time. (B) Kratky plot representation of the scattering data from RNA samples prepared by HS native method.
