
E. coli tRNAThrUGU with a box enclosing the four T-stem base pairs that were mutated in this work. The expected (Czerwoniec et al. 2009; Björk 1995) post-transcriptional modifications are as follows: D, dihydrouridine; T, 5-methyluridine; Ψ, pseudouridine; m7G, 7-methylguanosine, cmo5U, uridine 5-oxyacetic acid; t6A, N6-threonylcarbamoyladenosine.










