FIGURE 3.
Combined inhibition of let-7 and miR-34a reveals greater translational regulation of MYC mRNA. (A) Schematic of binding sites for miR-34a and let-7 in the MYC 3′ UTR (Kong et al. 2008; Kim et al. 2009a). Red sequence denotes miRNA seed regions, green denotes UTR. (B) Gradient distribution of MYC mRNA by coding-region-focused qRT–PCR, 17 h after transfection with anti-miRs against let-7 (red squares), miR-34a (orange triangles), both (purple diamonds), or a control (blue circles). Data shown are representative of two independent experiments.
