Translational readthrough of amber, opal, and ochre codons by tRNACys-derived suppressor tRNAs. Full-length AQP4-P mRNA containing an amber (A), opal (B) or ochre (C) codon at position 44 (indicated beneath gel) was translated in the presence of the corresponding suppressor tRNA (indicated above gel). Translation was carried out in WG extract in the presence of a Tran35S Label with or without an aptamer. Reactions were analyzed by SDS-PAGE and phosphorimaging. (D) mRNAs described in A were translated in WG extract in the presence of an aptamer and tRNA shown at the top of each lane. Translation reactions were analyzed by SDS-PAGE and phorphorimaging. The identity of the stop codon at residue 44 is indicated below the gel. “–,” Migration of polypeptides that terminate at residue 44; “*” and “↓,” migration of full-length AQP4-P protein.
