
(A) Schematic representation of the Azoarcus group I intron used in kinetic assays. The reverse K-turn sequence (boxed region) differs from a consensus K-turn at position A201. A TL-TLR interaction forms between P9 and J5/5a. Exogenous GMP is added in trans, and cleavage of the 5′ exon (represented by CAUAAA) is used to measure splicing activity. (B) Consensus K-turn sequence with G201. (C) Secondary structure of the base-paired construct.










