A fast selenium derivatization strategy for crystallization and phasing of RNA structures

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FIGURE 6.
FIGURE 6.

Experimental MAD electron density map (contoured at 1.5 σ level) obtained with the U2656-SeCH3 SRL RNA. (A) Stereo view showing residues 2651–2669. (B) Detailed view of the A2657-G2664 mismatch. Electron density maps of similar quality were obtained by SAD phasing with U2656-SeCH3 RNA, and by MAD/SAD phasing with the U2650-SeCH3 SRL sequence.

This Article

  1. Published in Advance February 18, 2009, doi: 10.1261/rna.1499309 RNA 15: 707-715

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