A fast selenium derivatization strategy for crystallization and phasing of RNA structures

Sequences and crystallization results obtained for the HIV-1 genomic RNA dimerization initiation site extended duplex bound to ribostamycin aminoglycoside (Freisz et al. 2008) according to the scheme proposed on Fig. 2. On the unmodified sequence (①), green and red arrows indicate 2′-OH positions available for 2′-OCH₃/SeCH₃ substitution or involved in crystal packing, respectively. The distance to the closest atom is indicated in green for available positions, and the number of hydrogen bonds involved in the packing interaction is shown in red.