Probing the architecture of the B. subtilis RNase P holoenzyme active site by cross-linking and affinity cleavage

Summary of cross-linking and AOP cleavage experiments. Ribbon diagram of the B. subtilis P protein (Stams et al. 1998) showing regions where AzP- or AOP-modified single-cysteines cross-link to or cleave PRNA and pre-tRNA (Table 2). Highlighted amino acids indicate positions where modification results in cross-linking/cleavage of PRNA only (green), pre-tRNA only (red), or both pre-tRNA and PRNA (blue). Labels indicate the location of the RNR motif (RNR), metal binding loop (MBL) and central cleft (CC).