Probing the architecture of the B. subtilis RNase P holoenzyme active site by cross-linking and affinity cleavage

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FIGURE 2.
FIGURE 2.

Cross-linking between P protein and PRNA. Cross-linking of B. subtilis RNase P holoenzyme reconstituted with equimolar-radiolabeled PRNA, pre-tRNA and AzP-labeled single-cysteine P protein variants (Niranjanakumari et al. 1998b). Lower mobility bands indicative of P protein–PRNA cross-linked species are indicated (XL).

This Article

  1. Published in Advance February 13, 2007, doi: 10.1261/rna.308707 RNA 13: 521-535

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