Evolutionary specialization of recoding: Frameshifting in the expression of S. cerevisiae antizyme mRNA is via an atypical antizyme shift site but is still +1

  1. IVAYLO P. IVANOV1,2,
  2. RAYMOND F. GESTELAND2, and
  3. JOHN F. ATKINS1,2
  1. 1BioSciences Institute, University College Cork, Cork, Ireland
  2. 2Department of Human Genetics, University of Utah, Salt Lake City, Utah 84112–5330, USA

Abstract

An autoregulatory translational shift to the +1 frame is required for the expression of ornithine decarboxylase antizyme from fungi to mammals. In most eukaryotes, including all vertebrates and a majority of the studied fungi/yeast, the site on antizyme mRNA where the shift occurs is UCC-UGA. The mechanism of the frameshift on this sequence likely involves nearly universal aspects of the eukaryotic translational machinery. Nevertheless, a mammalian antizyme frameshift cassette yields predominantly −2 frameshift in Saccharomyces cerevisiae, instead of the +1 in mammals. The recently identified endogenous S. cerevisiae antizyme mRNA has an atypical shift site: UGC-GCG-UGA. It is shown here that endogenous S. cerevisiae antizyme frameshifting is +1 rather than −2. We discuss how antizyme frameshifting in budding yeasts exploits peculiarities of their tRNA balance, and relate this to prior studies on Ty frameshifting.

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  1. Published in Advance January 23, 2006, doi: 10.1261/rna.2245906 RNA 12: 332-337 Copyright 2006 by RNA Society

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