Supplementary Data S1 – Identfied spliceosomal snRNA sequences in six diplonemid Species
This supplementary file contains the nucleotide sequences of conserved spliceosomal small nuclear RNAs (snRNAs) — including U1, U2, U4, U5, and U6 — from six diplonemid species:

Artemidia motanka (A. motanka)
Namystynia karyoxenos (N. karyoxenos)
Sulcionema specki (S. specki)
Lacrimia lanifica (L. lanifica)
Diplonema japonicum (D. japonicum)
Rhynchopus humris (R. humris)
Each entry includes:
A sequence identifier (e.g., >U1_C1_Dj), where:U1–U6 indicates the type of snRNA,C1, C2, etc., indicate multiple variants (when present),the last two-letter code refers to the species abbreviation.


A. motanka

>U2_Am
AUACCUUCUCGGCCUUUUGGCUAAGAUCAAGUGUAGUAUCUGUUCUUAUCAGCUUAACAU
CUGAUAGCACCCCUUGUGGGUGUUGAUGUUAUGAGAAUUUCUUGUGGGGGCGGCAGACAC
CCAGCGCAAGCUGGGCCUGCUCGCGAGUGUGGCCGCCAGGCUGCACCACACUGGUGCGCU
GCACACCCCUC
>U4_Am
AUCCUUGCGCUCGGGAAGUAUCCACAGGCAAUGACCUUCUUGGGACGCCGUGGAGAGAUU
GCUGGUUGAAAACUGCCCAAUUCCCCGCCCAGCGGCACUUCGGUGUGGCUGUGGCAAUUU
GUGGAACCACCAAAAGGUG
>U5_Am
ACUCACUCAGUCGACUCACAGCGAACCCAUCUUUCGCCUUUUACUAAAGAUGGCCGUGUG
UCGGCAGAAAAAGUGGACUGAGCACUCUUUGGAGCCCGCGAGGGU
>U6_Am
GCCCCGAAAGGGGACAUCCGCUAAAACCGGAACGAUACAGAGAAGAUCAGCAUGGUCCCU
GCGCAAGGAUGACACGCACUAAUCGAGAAAGUAUCACCUUUUUU

N. karyoxenos

>U1_Nk
GUACAUACCUGAUUGGUGUCCGGCCUGAUCAAACAGGGAGCCGACAUACCAGCGGUCCAC
GCAUUGCACUCCGCGUGGAACGUUGGUAGGAGCUGGGCUCUGCCUGGUCUCCACAAUGCA
AUUUUUGUCAGCGGUGGUGCUGCGCUUGCGCAAAUCCACCU
>U2_Nk
AUACCUUCUCGGCCUUUUGGCUAAGAUCAAGUGUAGUAUCUGUUCUUAUCAGCUUAAUAU
CUGAUAGCACUCUUCGUGAGUGCUGAUAUUUGACAAAUUUGUCCUUGGGGCAGUGGGCCG
CCCAGCGCAAGCUGGGUCCACAGCAGGUGUGGCCACUGAGCUUCACAACAUCAGAGUGCU
GCACACCCAUU
>U4_Nk
AUCCUUGCGCUCGGGAAGCAUCCACUGGCAAUGACCUUACUGGGAUGCCGUGGAGAGAUU
GCUGGUUGAAAACUACCCAAUUCCUAGUCUGGCCCCGCUGAGGUGGGGCACAGGCAAUUU
GUGGCACUGCCAAACGGCA
>U5_Nk
CUUCUGGCUCACGCUCAUUCCGAACUCAUCUUUCGCCUUUUACUAAAGAUGACCGUGGGC
GUGAGAGAACAGGACAGCUCAAGUCUUUUUUGUAGGGGCAGCGCCCCAUAA
>U6_Nk
GCCCAGCAAUGGGACAUCCGCUAAAAUUGGAACGAUACAGAGAAGAUUAGCAUGGUCCCU
GCGCAAGGAUGACACGCACUAAUCGAAAAUGUAUUCCCUUAUU

S. specki

>U1_C1_Ss
ACCCCCCCCUGAUUGGUGUCCGGCCUGAUCAAGUAGGGGGCCGACAUACGCGUGGUCCUU
UCAUUGCACUUCGAUAGGAACACGUGUAGGAGCUGGGCUCUGCCUGGUCUCCACAAUGUA
AUUUUUGGUAGCGGUGGUGCUGCGCUUGCGCAAACCCACCU
>U1_C2_Ss
GUACAUACCUGAUUGGUGUCCGGCCUGAUCAAGUAGGGAGCCGACAUACGCGUGGUCCUU
UCAUUGCACUUCGAUAGGAACACGUGUAGGAGCUGGGCUCUGCCUGGUCUCCACAAUGUA
AUUUUUGGUAGCGGUGGUGCUGCGCUUGCGCAAACCCACCU
>U2_Ss
AUACCUUCUCGGCCUUUUGGCUAAGAUCAAGUGUAGUAUCUGUUCUUAUCAGCUUAAUAU
CUGAUACGACGACUUGUUCGUCGAAUGAUAUUAACCUAAUUUCUUGCUGGGGUGCUGGGG
AGCUCUGGGCAACCAGAGUCCAGCACACGUGUUGCCCUGGAGCUGCACAACUUCCAGGCG
CGGCACACCCACU
>U4_Ss
AUCCUUGCGCUCGGGAAGUAUCCACAGGCAAUGACCUCACAGGGACGCCGUGGAGAGAUU
GCUGGUUGAAAACUACCCAAUUCCCCGCCGCUCGAUGCCUUCGGGCACGUUCGGCAAUUU
CUGCAACCGCCUCUAGGCG
>U5_Ss
GUCACGGUGCUUGCUCAUUCCGCAUUCAUCUUUCGCCUUUUACUAAAGAUGACCGUGGGC
AAGUGCCUCCGUGAAACAGCAUUUUUUGGAGGGUGCUUGCACCCU
>U6_Ss
GAACCCAGCAAUGGGACAUCCGUUAAAAUUGGAACGAUACAGAGAAGAUUAGCAUGGUCC
CUGCGCAAGGAUGACACGCACUAAUCGAGAAAGUAUCACCUUUUUC


L. lanifica

>U1_Ll
GUACAUACCUCAAUGGUGUCUGGCCUGAUCAAGUAGGGUGCCGACAUAUCAACGGUCCUU
GCAUUGCACUGCGCUCGGAACGUUGGUAGGGGCUGGUCUUCGACUGGCUUCCACAUUGAA
AUUUUUGGUAGCGGUGGUGCUGCGCUUGCGCAAACCCACCU
>U2_Ll
AUACCUUCUCGGCCUUUUGGCUAAGAUCAAGUGUAGUAUCUGUUCUUAUCAGCUUAAUAU
CUGAUAUGGCCCCUUCGGGGGUCAAGAUAUUAAACUAAUUUCUCGUGUGGGGUGGCAGGU
CGCUCGGGGCAACCCGAGUCUGUCCACAGGUGUUGCCCUGGAGUUGCACAGCAUCCAGGA
GCGGCACACCCAAA
>U4_Ll
AUCCUUGCGCUCGGGAUGAUCCACAGACUAUGACCGCUUAGGGAUGUCGUGGAGAGAUUG
CUGGUUGAAAACUACCCAAUUCCCCGUCGAGCGCUGAUUCGUCAGUGCUCAGACAAUUUC
UGGAACCGCCUCGGCG
>U5_Ll
UCUCACGAUCUUGGUUCAUCACGAACUCAUCUUUCGCCUUUUACUAAAGAUGACCGUGGA
CCUUGGUCAACAAGUGAUUGCACAAUUUUUCGAAAGGCCUUGAGCCUU
>U6_Ll
AGCCUCUUCGGAGGACAUCCGUUAAAAUUGGAUCGAUACAGAGAAGAUUAGCAUGGUCCC
UGCGCAAGGAUGACACGCAUUAAUCGAGAAAGUAUCACCUUUUUU

D. japonicum

>U1_C1_Dj
GUACAUACCUGAUUGGUGUCUGGCCUGAUCAAGUAGGGUGCCGACAUAUCAACGGUCCUU
GCAUUGCACUUCGCUUGGAACGUUGGUAGGGGCUGGGCUUGCCUGGCUUCCACAAUGAAA
UUUUUGGUAGCGGUGGUGCUGCGCUUGCGCAAACCCACCU
>U1_C2_Dj
GUACAUACCUGAUUGGUGUCUGGCCUGAUCAAGUAGGGUGCCGACAUAUCAACGGUCCUU
GCAUUGCACUUCGCUUGGAACGUUGGUAGGGGCUGGGCUUGCCUGGCUUCCACAAUGAAA
UUUUUGAUAGCGGUGGUGCUGCGCUUGCGCAAACCCACCU
>U1_C3_Dj
GAUCUUACCUGAUUGGUGUCUGGCCUGAUCAAGUAGGGUGCCGACAUAUCAACGGUCCUU
GCAUUGCACUUCGCUUGGAACGUUGGUAGGGGCUGGGCUUGCCUGGCUUCCACAAUGAAA
UUUUUGGUAGCGGUGGUGCUGCGCUUGCGCAAACCCACCU
>U2_Dj
AUACCUUCUCGGCCUUUUGGCUAAGAUCAAGUGUAGUAUCUGUUCUUAUCAGCUUAAUAU
CUGAUACAGCACCUCGUGUGCUGCGAUAUUAAACUAAUUUGUCGUUGGGGUGGCAGGCAG
CUCAGGGCAACCUGAGUCUGUCCACAGGUGUUGCCCUGGAGCUGCACAACAUCCAGGAGU
GGCACACCCUAC
>U4_Dj
AUCCUUGCGCUCGGGAAGAAGCCAUAGGCAAUGACCUAACAGGGAUGCCAUGGUGAGAUU
GCUGGUUGAAAACUACCCAAUUACCCGCUUGUCAGCACUACGGUGCUGUUCAAGCAAUUU
CUCAAGCUGCUUCUGAGCA
>U5_Dj
UCUCACGAUUCUUGUUCAUAACGAACUCAUCUUUCGCCUUUUUACUAAAGAUGACUGUGA
ACGUGGAUUAUUAAGUGAUAACGUAAAUUUUUUUAACAGGGGUCUUUUACCUUUCU
>U6_Dj
GUCCAGAAAUGGAACAUCCGUUAAAAUUGGAUCGAUACAGAGAAGAUUAGCAUGGUCCCU
GCGCAAGGAUGACACGCAUUAAUCGAGAAAGUAUCACCUUUUUU

R. humris
>U1_Rh
AUACCUGAUUGGUGUCAAGCCUGAUCACAAAGGGAGCUGACACACCAGCGGUCCUCGCAU
UGCACAACGCUUGGAACGUUGGUGGGAGCUGGUUUCGGCCGGUCUCCACAAUGUAAUUUU
UGGUCGCGGUGGUGCUGCGCUUGCGCAAAUCCACCU
>U2_Rh
AUACCUUCUCGGCCUUUUGGCUAAGAUCAAGUGUAGUAUCUGUUCUUAUCAGUUUAAUAU
CUGAUACAGUACCUCGUGUACUGAGAUAUUAUCCUAAUUUCUCACUGGGGUGGCGGGUCG
CUUGGGCAACCAAGUCCGCUCACAGGUGUUGCCCUGGAGCUGCACAACAUCCAGGAGUGG
CACACCCAAC
>U4_Rh
AUCCUUGCGCUCGGGAAGAAUCUACAGACAAUGACCAAUUCGGGAUGUCGUAGAGAGAUU
GCUGGUUGAAAACUACCCAAUACCCGUCUGCCUUCACUUCGGUAAAGGUUCAGACAAUUU
CUGGAGCUGCCUAGGCA
>U5_Rh
UCUCACGACCCUUGUUCAUAUCGAACUCAUCUUUCGCCUUUUACUAAAGAUGACCGUGGA
CAAGGAUCACUAAGUGAACAUAUAAUUUUUUAAGGGGCCUAGUGUCUCUA
>U6_Rh
GCCCAGAAAUGGGACAUCCGUUAAAAUUGGAUCGAUACAGAGAAGAUUAGCAUGGUCCCU
GCGCAAGGAUGACACGCAUUAAUCGAGAAAGUAUCACCUUUUUU