sample Figure Cell line size selection (nt) % mapping to CDS fold increase over undepleted undepleted 1 1B HEK293T 15-40 6.52 undepleted 2 1B HEK293T 15-40 6.72 legacy Ribo-Zero 1 1B HEK293T 15-40 16.39 2.48 legacy Ribo-Zero 2 1B HEK293T 15-40 16.86 2.55 riboCop 1 1B HEK293T 15-40 9.70 1.47 riboCop 2 1B HEK293T 15-40 9.87 1.49 NEBNext 1 1B HEK293T 15-40 11.47 1.73 NEBNext 2 1B HEK293T 15-40 10.11 1.53 undepleted 20-34 1D K562 20-34 6.16 NEBNext 20-34 1D K562 20-34 11.42 1.85 undepleted 20-60 1D K562 20-60 2.16 NEBNext 20-60 1D K562 20-60 5.45 2.52 undepleted 1 1F K562 20-34 6.76 undepleted 2 1F K562 20-34 7.11 ribozero plus 1F K562 20-34 4.80 0.69 "ribozero plus, o" 1F K562 20-34 2.22 0.32 "ribozero plus, f" 1F K562 20-34 1.69 0.24 "ribozero plus, of" 1F K562 20-34 1.54 0.22 QIAseq FastSelect 1 1F K562 20-34 7.33 1.06 QIAseq FastSelect 2 1F K562 20-34 7.16 1.03 riboPOOL 1 1F K562 20-34 6.80 0.98 riboPOOL 2 1F K562 20-34 6.59 0.95 "riboPOOL, no heating 1" 1F K562 20-34 6.68 0.96 "riboPOOL, no heating 2" 1F K562 20-34 6.77 0.98 undepleted 4A HEK293T 18-34 5.69 oligo_depletion 4A HEK293T 18-34 8.67 1.52