DNMT1 inhibition by pUG-fold quadruplex RNA
- Linnea I Jansson-Fritzberg1,
- Camila I Sousa2,
- Michael J Smallegan1,
- Jessica J Song1,
- Anne R Gooding1,
- Vignesh Kasinath1,
- John L Rinn1 and
- Thomas R Cech3,4
- 1 University of Colorado Boulder;
- 2 University of California Berkeley;
- 3 Howard Hughes Medical Institute, University of Colorado BioFrontiers Institute
- ↵* Corresponding author; email: cecht{at}hhmi.org
Abstract
Aberrant DNA methylation is one of the earliest hallmarks of cancer. DNMT1 is responsible for methylating newly replicated DNA, but the precise regulation of DNMT1 to ensure faithful DNA methylation remains poorly understood. A link between RNA and chromatin-associated proteins has recently emerged, and several studies have shown that DNMT1 can be regulated by a variety of RNAs. In this study we have confirmed that human DNMT1 indeed interacts with multiple RNAs, including its own nuclear mRNA. Unexpectedly, we found that DNMT1 exhibits a strong and specific affinity for GU-rich RNAs that form a pUG-fold, a non-canonical G-quadruplex. We find that pUG-fold-capable RNAs inhibit DNMT1 activity by inhibiting binding of hemimethylated DNA, and additionally provide evidence for multiple RNA binding modes with DNMT1. Together, our data indicates that a human chromatin-associated protein binds to and is regulated by pUG-fold RNA.
Keywords
- Received October 14, 2022.
- Accepted December 12, 2022.
- Published by Cold Spring Harbor Laboratory Press for the RNA Society
This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.
