Interactions between SAM and the 5’ UTR mRNA of the sam1 gene regulate translation in S. pombe
- ↵* Corresponding author; email: aihm{at}fudan.edu.cn
Abstract
The 5′ untranslated region (5’ UTR) of eukaryotic mRNA plays important role in translation. Here we report the function of the 5′ UTR mRNA of S-adenosylmethionine synthetase (sam1) in translational modulation in the presence of SAM in fission yeast Schizosaccharomyces pombe. Reporter assays, binding and chemical probing experiments and mutational analysis show that the 5′ UTR mRNA of sam1 binds to SAM to effect translation. Translational modulation is dependent on a tertiary structure transition in the RNA upon SAM binding. The characterization of such an RNA that is directly associated with an essential metabolic process in eukaryotes provides additional evidence that ligand binding by RNAs play important roles in eukaryotic gene regulation.
Keywords
- Received August 22, 2019.
- Accepted October 14, 2019.
- Published by Cold Spring Harbor Laboratory Press for the RNA Society
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