Real-time fluorescence detection of exoribonucleases
- 1Institut de Biologie Physico-Chimique, CNRS UPR 9073, Université de Paris 7–Denis Diderot, 75005 Paris, France
- 2Department of Biological Sciences, Columbia University, New York, New York 10027, USA
Abstract
The identification of RNases or RNase effectors is a continuous challenge, particularly given the current importance of RNAs in the control of genome expression. Here, we show that a fluorogenic RNA–DNA hybrid is a powerful tool for a real-time fluorescence detection and assay of exoribonucleases (RT-FeDEx). This RT-FeDEx assay provides a new strategy for the isolation, purification, and assay of known and unknown exoribonucleases.
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Footnotes
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Reprint requests to: Lionel Bénard, Institut de Biologie Physico-Chimique, CNRS UPR 9073, Université de Paris 7–Denis Diderot, 13 rue Pierre et Marie Curie, 75005 Paris, France; e-mail: lionel.benard{at}ibpc.fr; fax: (33)-1-58-41-50-20.
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Article published online ahead of print. Article and publication date are at http://www.rnajournal.org/cgi/doi/10.1261/rna.1670909.
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- Received March 31, 2009.
- Accepted August 12, 2009.
