Multiple independent domains of dGW182 function in miRNA-mediated repression in Drosophila

  1. Marina Chekulaeva1,2,
  2. Witold Filipowicz2 and
  3. Roy Parker1
  1. 1Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721, USA
  2. 2Department of Epigenetics, Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland

    Abstract

    miRNA-mediated repression affects a wide range of biological processes including development and human pathologies. The GW182 protein is a key component of miRNA repression complex, recruited by Argonaute and functioning downstream to repress translation and accelerate mRNA degradation, but little is known about how GW182 proteins act. Using both tethered function and complementation assays, we identify three independent domains of the Drosophila GW182 protein (also termed Gawky) that are sufficient to repress mRNA. Each of these domains also functions independently of poly(A) tails. These results indicate that miRNA-mediated repression is facilitated by multiple domains of GW182.

    Keywords:

    Keywords

    Footnotes

    • Reprint requests to: Marina Chekulaeva, Department of Epigenetics, Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland; e-mail: chekulae{at}fmi.ch; fax: 41-61-6973976; Witold Filipowicz, Department of Epigenetics, Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland; e-mail: Witold.Filipowicz{at}fmi.ch; or Roy Parker, Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721, USA; e-mail: rrparker{at}email.arizona.edu.

    • Article published online ahead of print. Article and publication date are at http://www.rnajournal.org/cgi/doi/10.1261/rna.1364909.

      • Received September 15, 2008.
      • Accepted January 22, 2009.

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    1. Published in Advance March 20, 2009, doi: 10.1261/rna.1364909 RNA Copyright © 2009 RNA Society

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