TABLE 2.
Plasmids
| Name | Features/construction | Source |
|---|---|---|
| pGEM7zf+ | Standard cloning vector | Promega |
| pGEMSLC1r | BamHI-KpnI PCR fragment of DYN2 inserted into pGEM7Zf+ | This work |
| pGAC14 | ACT1-CUP1 splicing reporter, GPD promoter; TRP1; 2u | Lesser and Guthrie 1993 |
| pGAC14Uf | URA3 gene insertion into Bg1II site of TRP1 gene of pGAC14 | This work |
| pGSC1t | pGAC14 ACT1 replaced by DYN2 frag.; CUP1 in E2+ frame | This work |
| pGSC1u | Same as pGSC1t except pGAC14Uf is parental plasmid | This work |
| pGSC3t | pGSC1t KpnI (blunted), religated; CUP1 in E2− frame | This work |
| pGSC3u | pGSC1u KpnI (blunted), religated; CUP1 in E2− frame | This work |
| pGSC621 | pGSC1t with the I1ΔBP mutation in DYN2 | This work |
| pGSC431 | pGSC1t with the I2Δ5′ss mutation in DYN2 | This work |
| pU[−2]C | DYN2 intron 1 branchpoint sequence U[−2]C; E2− frame | This work |
| pU[−1]A | DYN2 intron 1 branchpoint sequence U[−1]A; E2− frame | This work |
| pU[−1]A* | Same as pU[−1]A except in E2+ frame | This work |
| pU[−1]G | DYN2 intron 1 branchpoint sequence U[−1]G; E2− frame | This work |
| pU[1]A | DYN2 intron 1 branchpoint sequence U[1]A; E2− frame | This work |
| pA[2]G | DYN2 intron 1 branchpoint sequence A[2]G; E2− frame | This work |
| pGSlacIAt | pU[−1]G carrying the lac operator in intron 2 | This work |
| pRS316 | Empty vector control; URA3; CEN | Sikorski and Hieter 1989 |
| pJD4 | PPR2; URA3; CEN | Davie and Kane 2000 |
| pFL44D | Empty vector control; URA3, 2u | Archambault et al. 1992 |
| pPPR2 | PPR2 gene inserted into pFL44D | Archambault et al. 1992 |
