Perturbation of transcription elongation influences the fidelity of internal exon inclusion in Saccharomyces cerevisiae

Rescue of exon skipping by treatment with inhibitors of nucleotide biosynthesis. (A) Primer extension reactions of RNAs isolated from untreated (lanes 1,2) cells and from cultures treated with 6AU (lanes 3,4) or MPA (lanes 5,6). Reactions were normalized to the signal for SCR1. (B) Statistical analysis of dyn2 cDNA signals and ratios of exon skipping-to-inclusion. The 1-h or 2-h E2⁺, E2⁻, or E2⁻/E2⁺; quantities were compared with the 1-h or 2-h MOCK treatment, respectively. The 2-h MOCK was compared with the 1-h measurement. P value indicates the likelihood that the differences between the compared values are due to sampling error.