TABLE 1.
Comparison of programmed ribosomal frameshifting efficiencies obtained from monocistronic versus bicistronic frameshift reporter plasmid systems
| Genetic background: | JD1090 (rpl3Δ) | XY5a (rpl41A Δ rpl41B Δ) | ||||||
|---|---|---|---|---|---|---|---|---|
| Reporter | pRPL3 | pmak8 | pW255C | p257S | pI282T | pRS314–RPL41A | pRS314 | |
| L-A directed programmed −1 and Tyl directed programmed +1 frameshifting were determined in isogenic rpl3Δ or rpl41AΔ rpl41BΔ strains harboring the indicated plasmid encoded alleles of rpl3 or RPL41A using either lacZ-based monocistronic or dual-luciferase-based bicistronic reporter plasmid systems. | ||||||||
| N.D.: Not determined. Values for percent programmed ribosomal frameshifting (PRF) and standard deviations are shown. All experiments were performed three times in triplicate. | ||||||||
| % −1 PRF | Monocistronic | 1.90 ± 0.09 | 4.76 ± 0.14 | 4.54 ± 0.10 | 4.50 ± 0.05 | 3.82 ± 0.12 | 1.7% ± 0.05 | 2.9 ± 0.11 |
| Bicistronic | 9.0 ± 0.4 | 14.5 ± 0.2 | 14.2 ± 1.2 | 13.9 ± 1.3 | 13.7 ± 0.6 | 13.1 ± 0.6 | 20.0 ± 1.9 | |
| % +1 PRF | Monocistronic | 12.6 ± 0.2 | 13.8 ± 0.6 | 12.3 ± 0.7 | 11.3 ± 1.0 | 11.7 ± 1.0 | N.D. | N.D. |
| Bicistronic | 8.5 ± 0.4 | 7.7 ± 0.4 | 7.8 ± 0.8 | 8.8 ± 0.6 | 9.1 ± 1.1 | 20.0 ± 0.8 | 19.9 ± 0.4 | |
