Ribosomes containing mutant forms of L3 have decreased peptidyltransferase activities. Time course of the formation of [14C]Phenylalanine-puromycin product in assays using ribosomes isolated from isogenic RPL3|mBHIS3 cells expressing wild-type or mutant forms of L3. Plasmid-borne RPL3 alleles are indicated. EtOAc soluble radioactivity was determined by liquid scintillation counting. Control studies were performed in the absence of puromycin to determine the nonspecific extraction of CACCA[14C]AcPhe. Control values (generally less than 2%) were subtracted from the values obtained in the presence of puromycin. All experiments were performed in triplicate. Data points and error bars indicate mean and standard deviation.
