Stereospecificity of short Rev-derived peptide interactions with RRE IIB RNA

RRE IIB footprinting studies of the Rev peptides binding sites on the RNA construct. (A) Lead acetate footprinting. (B) RNase T1 footprinting. (C) RNase A footprinting. (0) Untreated RRE IIB, ³²P-labeled at 5′; (Ctrl) control experiment in the absence of binders; (L-Rev, D-Rev, L-RevErse, D-RevErse) RNA footprinting in the presence of peptide binders; (NeoB) RNA footprinting in the presence of neomycin B. Concentrations of the peptides, 2.5 and 12.5 μM. Concentration of neomycin B 25 and 125 μM.