FIGURE 4.
Phylogenetic tree of known Sec and Trp tRNAs. Sequences used to generate the tree were extracted from nonredundant and microbial databases at NCBI or were obtained as described in Fig. 3 and its legend. Nucleotide sequence alignments were generated using the PileUP program. Distances between sequences were calculated from these alignments by the DISTANCE program with the Kimura distance-measuring methods. Finally, unrooted phylogenic trees were visualized by the GrowTree program. The trees were used to trace the evolutionary origin of Sec tRNAs.
