The G2447A mutation does not affect ionization of a ribosomal group taking part in peptide bond formation

Effect of the G2447A mutation on the peptidyl transferase reaction. Posttranslocation complexes of G2447A (squares) or wild-type (triangles) ribosomes were reacted with Pm at 37°C (Materials and Methods). Values are normalized by setting to 1.0 the maximum extent of fMetPhe-Pm formed per posttranslocation complex present. Actual values were 0.8–0.9 for wild-type and 0.5–0.6 for mutant ribosomes.