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Considering the presence or absence of four independent catalytic strategies results in 16 possible combinations for enzymatic mechanism. The first entry is the state of no catalysis and is the background rate constant from which the remaining speed limits are calculated (23°C, 250 mM K+). Speed limits are the predicted rate constants for perfect use of the indicated catalytic combination. The highlighted speed limit is the common αγ speed limit. Asterisks (*) denote those speed limits for δ catalysis without β catalysis that are predicted not to be chemically permitted. Exceptions to this prediction might be permitted if an enzyme can metalate the 5′ oxygen without regard for the protonation or metalation state of the NBPO.
