FIGURE 4.
Antisense oligonucleotide-mediated activation and suppression of 1–561 RNA tight dimerization. 1–561 RNA was incubated for 30 min at 55°C in dimer buffer with a 20-fold excess of as202, as548, asDIM, or a combination of these oligonucleotides and subjected to electrophoresis on TBE agarose gel at 28°C. Antisense DNA oligonucleotides as202 and as548 target the upstream and downstream dimer-interfering elements, respectively, previously characterized in our laboratory (Lanchy et al. 2003). asDIM is complementary to nt 397–426 and inhibits SL1-dependent tight dimerization of the 1–444 model RNA (Fig. 2).
