RNA-binding activity of translation initiation factor eIF4G1 from Saccharomyces cerevisiae

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FIGURE 6.
FIGURE 6.

Filter-binding assay. The titration curves of three of the GST-eIF4G1 fusion proteins tested are shown on the left. Proteins were titrated against 0.25 pmole [32P|-labeled 38-nt RNA in a 20-μL reaction volume containing buffer A. KDapprox. values for GST-eIF4G1 fusion proteins are shown on the right.

This Article

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