RNA-binding activity of translation initiation factor eIF4G1 from Saccharomyces cerevisiae

In vivo phenotype of eIF4G1 deletion mutants. (A) Schematic representation of yeast eIF4G1 and eIF4G1 fragments. Pab1, poly(A) binding protein binding domain; 4E, eIF4E-binding domain; RS, arginine- and serine-rich domains; 4A, eIF4A-binding domain. (B) The phenotype observed on plates of yeast strains expressing the various eIF4G1 deletion proteins as only source of eIF4G1 (either under the Gal1/10 or the eIF4G promoter) is indicated. wt, wild-type growth; ts, temperature sensitive (no growth at 37°C, normal growth at 30°C).