Overexpression of UAP56 reduces GFP expression. (A) Western blot showing overexpression of UAP56 following heat shock in BL7040 transgenic worms. Equivalent numbers of worms of the same stage were prepared for the heat-shocked and non-heat-shocked samples 2 h after heat shock. (B) Heat-shock-induced UAP56 expression is abundant in embryos and intestine. Immunofluorescent staining of wild-type (WT) and heat-shocked BL7040 worms reacted with anti-UAP56 antibody. (C) BL7042 worms transgenic for HS:UAP56 and for vit:gfp were heat-shocked at the L4 larval stage or as young adults. L4 heat-shocked and control worms were scored 20 h after the heat shock (as adults) for GFP expression. A separate population of worms was heat-shocked as young adults and scored at intervals (1, 4, and 20 h after heat shock). For each population of size n, the percent of animals scored at each level of expression is indicated by the size of the circles as in Figure 3.
