FIGURE 1.
Transgenic worms with the integrated pNvitgfp construct express GFP in adult hermaphrodite intestines. (A) For the construct, 247 bp of the vit-2 5′-flanking region (blue) is fused to vector sequences derived from pPD95.70 (black) that contain two synthetic introns that precede the gfp gene (green). The presence of an NLS directs the GFP to the nucleus. Part of the last exon of vit-6 provides the 3′-end formation site (unfilled box). This construct was injected into worms, and a transgenic strain, BL3466, was selected that carries multiple tandem copies of this construct integrated into the genome. (B) GFP expression in BL3466.
