TABLE 2.
Nonselective editing of backbone gus-PSTVd RNA constructs
| Segment number | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8L | 9 | 10 | |
| The numbers of editing sites in both upper and lower strands were combined unless indicated (L = lower strand only). Editing was defined as an unambiguous heterogenic A and G peak in the sequencing electropherogram. | ||||||||||
| gus-PSTV backbone substrate | ||||||||||
| No. edited sites | 0 | 0 | 2 | 1 | 2 | 5 | 6 | 0 | 0 | 0 |
| Total no. of As analyzed | 12 | 25 | 21 | 15 | 21 | 20 | 21 | 14 | 18 | 3 |
| Percent edited As | — | — | 10 | 7 | 10 | 25 | 29 | — | — | — |
| Wild-type R/G substrate | ||||||||||
| No. edited sites | 0 | 0 | 2 | 1 | 2 | 5 | 5 | 0 | 0 | 0 |
| Total no. of As analyzed | 12 | 25 | 21 | 15 | 21 | 20 | 21 | 14 | 18 | 3 |
| Percent edited As | — | — | 10 | 7 | 10 | 25 | 24 | — | — | — |
| G56 mutant substrate | ||||||||||
| No. edited sites | 0 | 0 | 3 | 1 | 2 | 5 | 6 | 0 | 0 | 0 |
| Total no. of As analyzed | 12 | 25 | 21 | 15 | 21 | 20 | 21 | 14 | 18 | 3 |
| Percent edited As | — | — | 14 | 7 | 10 | 25 | 29 | — | — | — |
