FIGURE 5.
Editing at the R/G site in the wild-type and G56 mutant substrates. A limited primer-extension assay was used to monitor the R/G site after incubation without rADAR2 (C) or with rADAR2. The assay was performed on the wild-type (wt R/G) and the G56 mutant (G56) substrates. Primer extension was done in the presence of ddT so that reverse transcriptase terminated at the R/G site for nonedited molecules and continued to the next adenosine if the R/G was edited. Editing efficiency was calculated as described previously in Öhman et al. (2000).
