The dynamic nature of the four-way junction of the hepatitis C virus IRES

TABLE 1.

Two-state model fits of FRET efficiency data for ion-induced folding of IRES and 4H junctions as a function of Mg2+ concentration

Vector [Mg]1/2/μM Error n Error
Values of [Mg]1/2 and Hill coefficients (n) for individual end-to-end vectors calculated from the fits shown in Figure 4, with standard errors.
4H junction
    BC 132 61 0.8 0.07
    SB 176 114 1.1 0.2
IRES junction
    CS 1.1 0.7 1.1 0.1
    BA 3 2 0.56 0.07
    AC 1.3 0.6 0.83 0.04
    BS 6 3 2.9 0.7
    AS 4 2 0.68 0.06
    BC 3 1 0.60 0.04

This Article

  1. RNA 9: 809-820