The dynamic nature of the four-way junction of the hepatitis C virus IRES

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FIGURE 4.FIGURE 4.
FIGURE 4.

Ion-induced folding of the junctions studied by the change in FRET efficiency as a function of Mg2+ concentration for particular vectors. (A) 4H junction: BC (open circle); SB (closed circle). (B) IRES junction: BA (closed circle); CS (open circle); BS (closed triangle); AC (open triangle); AS (closed square); BC (open square). The data have been fitted to two-state models (lines), from which [Mg]1/2 and Hill coefficients (n) have been calculated and given in Table 1.

This Article

  1. RNA 9: 809-820