FIGURE 3.
(A) Sequences of the template library (21 clones) after four rounds of selection on streptavidin-agarose. (B) Incorporation of biocytin via canonical and noncanonical base-pair interactions at GUA codons. Approximately 30 pmole of template with a fixed GUA codon was translated in the presence of 2.0 μg biocytin-acylated tRNAs containing the anticodon NAC, where the first position N is either U, C, A, or G. Values are expressed as the percent of [35S]Met-labeled mRNA–peptide fusions bound to immobilized streptavidin.
