Antagonistic signals within the COX2 mRNA coding sequence control its translation in Saccharomyces cerevisiae mitochondria

TABLE 1.

S. cerevisiae strains used in this study

Strain namea COX2 or COX3 allele Source or reference
aAll strains are ρ+ unless otherwise indicated.
bAll strains isogenic or congenic to DBY947 have the nuclear genotype MATα ade2-101 arg8Δ::URA3 leu2Δ ura3-52 kar1-1.
cAll strains isogenic or congenic to D273-10B have the nuclear genotype MATa arg8::hisG his3ΔHindIII leu2-3,112 lys2 ura3-52.
dThe designation “2–6*” denotes the introduction of silent mutations into the mRNA for codons 2–6.
eThe genotype for NSG171 ρ° is MATα ade2 arg8::hisG lys2 ura3Δ.
fThese strains contain a ρ+ genome with the indicated allele at the COX3 locus and the cox2-62 allele at the COX2 locus.
gThe genotype for TF236 is MATa arg8::hisG his3? lys2 ura3-52 inolΔ::HIS3 pet9 [cox3::arg8m-1 ρ+].
Strains isogenic or congenic to DBY947b
    DFS160 ρ° Steele et al. (1996)
    NB63 cox2–107 Bonnefoy and Fox (2000)
    NB71 cox3::arg8m-1 Bonnefoy and Fox (2000)
Strains isogenic or congenic to D273-10Bc
    NB40-3C cox2–62 Bonnefoy and Fox (2000)
    NB43 cox2(1–91)::ARG8m Bonnefoy and Fox (2000)
    NB80 COX2 Bonnefoy and Fox (2000)
    NB120 cox2(1,15–91)::ARG8m Bonnefoy et al. (2001)
    NB135 cox2(1–6,15–91)::ARG8m Bonnefoy et al. (2001)
    NB179 cox2(1,2–6*,15–91)::ARG8md Bonnefoy et al. (2001)
    NSG171 ρ°e COX2 N.S. Green-Willms (unpubl.)
    EHW154 cox2(1,89–91)::ARG8m This study
    EHW155 cox2(1,15–25,89–91)::ARG8m This study
    EHW156 cox2(1,15–35,89–91)::ARG8m This study
    EHW157 cox2(1,15–45,89–91)::ARG8m This study
    EHW158 cox2(1,15–55,89–91)::ARG8m This study
    EHW159 cox2(1,15–65,89–91)::ARG8m This study
    EHW160 cox2(1,15–75,89–91)::ARG8m This study
    EHW161 cox2(1,15–85,89–91)::ARG8m This study
    EHW185 cox2(1,15–24,89–91)::ARG8m This study
    EHW186 cox2(1,15–23,89–91)::ARG8m This study
    EHW187 cox2(1,15–22,89–91)::ARG8m This study
    EHW188 cox2(1,15–21,89–91)::ARG8m This study
    EHW189 cox2(1,15–20,89–91)::ARG8m This study
    EHW190 cox2(1,15–19,89–91)::ARG8m This study
    EHW191 cox2(1,15–18,89–91)::ARG8m This study
    EHW192 cox2(1,15–17,89–91)::ARG8m This study
    EHW193 cox2(1,15–16,89–91)::ARG8m This study
    EHW194 cox2(1,15,89–91)::ARG8m This study
    EHW195 cox2(1,16–25,89–91)::ARG8m This study
    EHW196 cox2(1,17–25,89–91)::ARG8m This study
    EHW197 cox2(1,18–25,89–91)::ARG8m This study
    EHW198 cox2(1,19–25,89–91)::ARG8m This study
    EHW199 cox2(1,20–25,89–91)::ARG8m This study
    EHW200 cox2(1,21–25,89–91)::ARG8m This study
    EHW201 cox2(1,22–25,89–91)::ARG8m This study
    EHW202 cox2(1,23–25,89–91)::ARG8m This study
    EHW203 cox2(1,24–25,89–91)::ARG8m This study
    EHW204 cox2(1,25,89–91)::ARG8m This study
    EHW328 cox2–230::ARG8m This study
    EHW329 cox2–231::ARG8m This study
    EHW330 cox2–232::ARG8m This study
    EHW334f cox3Δ::ARG8m This study
    EHW335f cox3–41 This study
    EHW336f cox3–42 This study
    EHW357 cox2(1,46–75,89–91)::ARG8m This study
    EHW358 cox2(1,22–45,89–91)::ARG8m This study
    EHW378f cox3–43 This study
    EHW379f cox3–44 This study
    EHW380 cox2(1–25,89–91)::ARG8m This study
    EHW383 cox2(1–6,15–25,89–91)::ARG8m This study
    EHW384 cox2(1,2–6*,15–25,89–91)::ARG8md This study
    EHW391f cox3–45 This study
    EHW392f cox3–46 This study
    EHW393f cox3–47 This study
    EHW409 cox2(1–14,46–75,89–91)::ARG8m This study
    EHW410 cox2(1–6,46–75,89–91)::ARG8m This study
    EHW411 cox2(1,2–6*,46–75,89–91)::ARG8md This study
    EHW412 cox2(1–14,22–45,89–91)::ARG8m This study
    EHW413 cox2(1–6,22–45,89–91)::ARG8 m This study
    EHW414 cox2(1,2–6*,22–45,89–91)::ARG8md This study
Mixed lineage strain
    TF236g cox3::arg8m-1 Bonnefoy and Fox (2000)

This Article

  1. RNA 9: 419-431