TABLE 1.
Bacterial strains and plasmids used in this study
| Strain/ plasmid | Relevant genotype and property | Source |
|---|---|---|
| Strain | ||
| PP47 | crp | Aiba et al. 1982 |
| PP47ssrA | PP47ΔssrA::Kan | Abo et al. 2000 |
| W3110 | Wild-type | Laboratory stock |
| TA331 | W3110ΔssrA::FRT | Abo et al. 2002 |
| TA371 | W3110ssrADD-FRT | Abo et al. 2002 |
| YA002 | PP47Δmr-FRT | This work |
| YA004 | PP47ΔssrA::Kan Δmr-FRT | This work |
| Plasmid | ||
| pHA7 | Derivative of pBR322 carrying the crp gene expressed from the bla promoter | Aiba et al. 1982 |
| pHA7M9 | Derivative of pHA7 in which the crp terminator is mutated by a 2-base substitution in the hairpin region | This work |
| pHA7M6 | Derivative of pHA7 in which the crp terminator is extensively mutated | Abe et al. 1999 |
| pHA7S1 | Derivative of pHA7 in which the TAA stop codon of crp was changed to AAA | Ueda et al. 2002 |
| pYY100 | Derivative of pBR322 carrying a variant crp encoding His6-CRP with the wild-type terminator | This work |
| pYY101 | Derivative of pYY101 in which the crp terminator is mutated by a 2-base substitution in the hairpin region | This work |
| pQE-80L | Expression vector for N-terminally His6-tagged protein | QIAGEN |
| pMelR | Derivative of pQE-80L carrying a variant melR encoding His6-MelR | This work |
| pSoxR | Derivative of pQE-80L carrying a variant soxR encoding His6-SoxR | This work |
