SsrA-mediated trans-translation plays a role in mRNA quality control by facilitating degradation of truncated mRNAs

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FIGURE 2.
FIGURE 2.

Effect of SsrA RNA on the expression of full-length CRP. Total extracts were prepared from PP47 ssrA+ and PP47 ssrA cells harboring pHA7 derivatives. Lysates equivalent to 0.005 OD600 units (Miller 1972) were analyzed by Western blotting using anti-CRP antibodies. The band of the full-length CRP (23.6 kD) is shown by an asterisk.

This Article

  1. RNA 9: 408-418