RNA aptamers to initiation factor 4A helicase hinder cap-dependent translation by blocking ATP hydrolysis

Base changes in the selected RNA that affect affinity for eIF4A. Base substitutions of RNA no. 20 are generated by mutagenic PCR. Experimental procedures are the same as in Figure 2. (A) Filter-binding profiles of moderately affected variants. (B) Filter-binding profiles of severely affected variants. (C) Base changes of moderately affected variants. (D) Base changes of severely affected variants. M8 and M15 are double mutants, whereas M10 is triple mutant.