Recognition of acceptor-stem structure of tRNAAsp by Escherichia coli aspartyl-tRNA synthetase

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 4.
FIGURE 4.

Method used to construct the randomized library of yeast tRNAAsp mutants. Two synthetic oligonucleotides were annealed, one round of complementary strands was synthesized by Taq DNA polymerase, and the product was cleaved by restriction enzymes. The shaded bases are the targets of mutagenesis, and the boxed residues at positions 1–5 are the sequence tags.

This Article

  1. RNA 9: 386-393

Current Issue

  1. August 2026, 32 (8)
  1. Current Issue
  1. Alert me to new issues of RNA